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Product Info Summary
| SKU: | A14164 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ZBTB3 Antibody
SKU/Catalog Number
A14164
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZBTB3 Antibody (Catalog # A14164). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ZBTB3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZBTB3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14164)
Host
Rabbit
Contents
ZBTB3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZBTB3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ZBTB3. The immunogen is located within amino acids 510 - 560 of ZBTB3.
Cross-reactivity
At least three isoforms of ZBTB3 are known to exist. This antibody is predicted to not cross-react with other ZBTB protein family members.
Reactive Species
A14164 is reactive to ZBTB3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
61.8 kDa
Background of ZBTB3
The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. While little is known about ZBTB3, the related protein ZBTB2 is thought to be phosphorylated in response to the DNA damage, probably by either ATM or ATR. Other ZBTB proteins, such as ZBTB4 and ZBTB38 bind methylated DNA and repress transcription, suggesting that ZBTB3 may also act as a transcription repressor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14164 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZBTB3 antibody can be used for detection of ZBTB3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZBTB3 in rat brain tissue lysate with ZBTB3 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of ZBTB3 in human brain tissue with ZBTB3 antibody at 2.5 μg/mL.
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Immunofluorescence of ZBTB3 in human brain tissue with ZBTB3 antibody at 20 μg/mL.
Specific Publications For Anti-ZBTB3 Antibody (A14164)
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