Anti-ZBTB5 Antibody

Product Name

Anti-ZBTB5 Antibody

View all ZBTB5 Antibodies

SKU/Catalog Number

A14543

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ZBTB5 Antibody (Catalog # A14543). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ZBTB5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ZBTB5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14543)

Host

Rabbit

Contents

ZBTB5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ZBTB5 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human ZBTB6. The immunogen is located within the last 50 amino acids of ZBTB5.

Cross-reactivity

At least two isoforms of ZBTB5 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.

Reactive Species

A14543 is reactive to ZBTB5 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

74.3 kDa

Background of ZBTB5

The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB5 was identified though sequence analysis as a POZ domain Kruppel-like zinc finger (POK) protein. Further experiments indicated that it binds DNA and can directly repress transcription of the cell cycle arrest gene p21. ZBTB5 can also interact with co-repressor histone deacetylase complexes such as BCoR, NCoR, and SMRT via its POZ domain, resulting in deacetylation of histones Ac-H3 and Ac-H4 at the proximal promoter. ZBTB5 stimulates both cell proliferation and cell cycle progression, suggesting that it may act as a potential proto-oncogene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14543 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ZTBT5 antibody can be used for detection of ZTBT5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ZBTB5

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