Product Info Summary
| SKU: | A14485 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ZBTB6 Antibody
SKU/Catalog Number
A14485
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZBTB6 Antibody (Catalog # A14485). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ZBTB6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZBTB6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14485)
Host
Rabbit
Contents
ZBTB6 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZBTB6 antibody was raised against a 15 amino acid synthetic peptide near the center of human ZBTB6. The immunogen is located within amino acids 180 - 230 of ZBTB6.
Cross-reactivity
At least two isoforms of ZBTB6 are known to exist; this antibody will only recognize the shorter isoform. This antibody is predicted to not cross-react with other ZBTB protein family members.
Reactive Species
A14485 is reactive to ZBTB6 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
48.2 kDa
Background of ZBTB6
The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB6, also known as ZNF482, is highly expressed in brain. While little is known about ZBTB6, other ZBTB proteins, such as ZBTB4 bind methylated DNA and repress transcription. Another ZBTB family member, ZBTB5, also acts as a transcriptional repressor, and can interact with co-repressor histone deacetylase complexes such as BCoR, NCoR, and SMRT via its POZ domain, suggesting that ZBTB6 may also act as a transcriptional repressor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14485 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZBTB6 antibody can be used for detection of ZBTB6 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZBTB6 in EL4 cell lysate with ZBTB6 antibody at 1 μg/mL.
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Immunocytochemistry of ZBTB6 in EL4 cells with ZBTB6 antibody at 5 μg/mL.
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Immunofluorescence of ZBTB6 in EL4 cells with ZBTB6 antibody at 20 μg/mL.
Specific Publications For Anti-ZBTB6 Antibody (A14485)
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