Anti-ZC3H7A Antibody Picoband™

Product Name

Anti-ZC3H7A Antibody Picoband™

View all ZC3H7A Antibodies

SKU/Catalog Number

A15075-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ZC3H7A Antibody Picoband™ catalog # A15075-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ZC3H7A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15075-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ZC3H7A recombinant protein (Position: Q13-R717).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A15075-1 is reactive to ZC3H7A in Human

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

111 kDa

Calculated molecular weight

19908 MW

Background of ZC3H7A

ZC3H7A (Zinc Finger CCCH-Type Containing 7A, also known as ZC3H7; HSPC055; ZC3HDC7), a member of CCCH gene family, is located on human chromosome 16p13.13. ZC3H7A is associated with some diseases, including Endometrial Stromal Tumor and Endometrial Stromal Sarcoma. An important paralog of this gene is ZC3H7B. The CCCH zinc finger motif has been found in proteins from organisms ranging from man to yeast. The CCCH proteins are a large family of zinc finger containing C3H-type motifs and much evidences proved that they may be RNA-binding proteins functioning in RNA processing. In mouse, tristetraprolin, a protein containing two CCCH zinc fingers, binds directly to AU-rich elements within the 3'-untranslated region of target transcripts to facilitate mRNA degradation. Zfp36l2, like its better-known relative TTP, is a mRNA-binding and destabilizing protein, functions in the physiological control of female fertility at the level of early embryonic development. The PIE-1 is an essential regulator of Caenorhabditis elegans germ cell fate that segregates with the germ lineage by inhibition of transcription or activation of protein expression from maternal RNAs.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A15075-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Positive Control

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ZC3H7A using anti-ZC3H7A antibody (A15075-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZC3H7A antigen affinity purified polyclonal antibody (Catalog # A15075-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZC3H7A at approximately 111 kDa. The expected band size for ZC3H7A is at 111 kDa.

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Figure 2. IF analysis of ZC3H7A using anti-ZC3H7A antibody (A15075-1).
ZC3H7A was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ZC3H7A Antibody (A15075-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of HL-60 cells using anti-ZC3H7A antibody (A15075-1).
Overlay histogram showing HL-60 cells stained with A15075-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZC3H7A Antibody (A15075-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For ZC3H7A (Source: Uniprot.org, NCBI)

Alternative Names

FLJ10027; FLJ20318; HSPC055; ZC3H7; ZC3HDC7; zinc finger CCCH domain-containing protein 7A; zinc finger CCCH type domain containing 7; zinc finger CCCH-type containing 7; zinc finger CCCH-type containing 7A; zinc finger CCCH-type domain containing 7; zinc-finger protein AY163807 ZC3H7A HSPC055, ZC3H7, ZC3HDC7 zinc finger CCCH-type containing 7A zinc finger CCCH domain-containing protein 7A|zinc finger CCCH-type domain containing 7|zinc-finger protein AY163807

For more info on ZC3H7A, check out the ZC3H7A Infographic

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In this infographic, you will see the following information for ZC3H7A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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