Anti-ZC3H7B Antibody

ZC3H7B antibody

Boster Bio Anti-ZC3H7B Antibody (Catalog # A10596). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A10596
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-ZC3H7B Antibody

View all ZC3H7B Antibodies

SKU/Catalog Number

A10596

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-ZC3H7B Antibody (Catalog # A10596). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ZC3H7B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10596)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human ZC3H7B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10596 is reactive to ZC3H7B in Human

Applications

A10596 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

109.858kDa

Background of ZC3H7B

a tetratricopeptide (TPR) protein containing 5 zinc fingers. Interacts with the rotavirus non-structural protein NSP3. TPR proteins are involved in a variety of biological processes, such as cell cycle regulation, transcriptional control, mitochondrial and peroxisomal protein transport, neurogenesis and protein folding.

Ota T., Nat. Genet. 36:40-45(2004).
Rush J., Nat. Biotechnol. 23:94-101(2005).
Kikuno R., DNA Res. 6:197-205(1999).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For ZC3H7B (Source: Uniprot.org, NCBI)

Gene Name

ZC3H7B

Full Name

Zinc finger CCCH domain-containing protein 7B

Weight

109.858kDa

Alternative Names

FLJ13787; Rotavirus 'X' associated non-structural protein; rotavirus X protein associated with NSP3; zinc finger CCCH-type containing 7B ZC3H7B ROXAN1, RoXaN zinc finger CCCH-type containing 7B zinc finger CCCH domain-containing protein 7B|Rotavirus X associated non-structural protein|rotavirus X-associated non-structural protein|rotavirus X protein associated with NSP3|ubiquitous tetratricopeptide containing protein RoXaN

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ZC3H7B, check out the ZC3H7B Infographic

ZC3H7B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ZC3H7B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-ZC3H7B Antibody

Question

We are currently using anti-ZC3H7B antibody A10596 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

The anti-ZC3H7B antibody (A10596) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-30

Question

I see that the anti-ZC3H7B antibody A10596 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-28

Question

My question regarding product A10596, anti-ZC3H7B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-16

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10596 anti-ZC3H7B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-16

Question

Please see the WB image, lot number and protocol we used for trachea using anti-ZC3H7B antibody A10596. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-18

Question

Will anti-ZC3H7B antibody A10596 work on feline WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

Our lab technicians have not tested anti-ZC3H7B antibody A10596 on feline. You can run a BLAST between feline and the immunogen sequence of anti-ZC3H7B antibody A10596 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline cervix carcinoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-16

Question

Will A10596 anti-ZC3H7B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-02-05

Answer

You can see on the product datasheet, A10596 anti-ZC3H7B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-02-05

Question

My question regards to test anti-ZC3H7B antibody A10596 on human trachea for research purposes, then I may be interested in using anti-ZC3H7B antibody A10596 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Mangal

Verified customer

Asked: 2015-04-10

Answer

The products we sell, including anti-ZC3H7B antibody A10596, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-04-10

Order DetailsPrice
A10596-100uL

100uL

$360

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