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Product Info Summary
| SKU: | AZA0A8M1RNN7 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish ABCB11B Antibody
SKU/Catalog Number
AZA0A8M1RNN7
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish ABCB11B Antibody catalog # AZA0A8M1RNN7. Tested in IHC applications.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish ABCB11B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A8M1RNN7)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish ABCB11B recombinant protein (Position: K6-D1236).
Reactive Species
AZA0A8M1RNN7 is reactive to ABCB11B in Zebrafish
Background of ABCB11B
Predicted to enable ABC-type bile acid transporter activity and canalicular bile acid transmembrane transporter activity. Acts upstream of or within bile acid secretion. Predicted to be located in membrane. Predicted to be active in apical plasma membrane. Is expressed in liver. Used to study progressive familial intrahepatic cholestasis 2. Human ortholog(s) of this gene implicated in benign recurrent intrahepatic cholestasis 2; intrahepatic cholestasis; liver cirrhosis; and progressive familial intrahepatic cholestasis 2. Orthologous to human ABCB11 (ATP binding cassette subfamily B member 11).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA0A8M1RNN7 is guaranteed for IHC Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Immunohistochemistry (Paraffin-embedded Section) | 2-5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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IHC analysis of ABCB11B using anti-ABCB11B antibody (AZA0A8M1RNN7).
ABCB11B was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCB11B Antibody (AZA0A8M1RNN7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish ABCB11B Antibody (AZA0A8M1RNN7)
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