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Product Info Summary
| SKU: | AZA2CG95-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish ACADM Antibody Picoband®
SKU/Catalog Number
AZA2CG95-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish ACADM Antibody Picoband® catalog # AZA2CG95-1. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish ACADM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA2CG95-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish ACADM recombinant protein (Position: S43-E406).
Reactive Species
AZA2CG95-1 is reactive to ACADM in Zebrafish
Observed Molecular Weight
43 kDa
Background of ACADM
ACADM (acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain) is a gene that provides instructions for making an enzyme called acyl-coenzyme A dehydrogenase that is important for breaking down (degrading) a certain group of fats called medium-chain fatty acids. This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA2CG95-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Positive Control
WB: zebrafish head tissue, female zebrafish viscera tissue, male zebrafish viscera tissue, whole zebrafish tissue, zebrafish embryo tissue
Validation Images & Assay Conditions
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Western blot analysis of ACADM using anti-ACADM antibody (AZA2CG95-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: female zebrafish viscera tissue lysates,
Lane 3: male zebrafish viscera tissue lysates,
Lane 4: whole zebrafish tissue lysates,
Lane 5: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACADM antigen affinity purified polyclonal antibody (AZA2CG95-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ACADM at approximately 43 kDa. The expected band size for ACADM is at 47 kDa.
Specific Publications For Anti-Zebrafish ACADM Antibody Picoband® (AZA2CG95-1)
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