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- Table of Contents
ACACA antibody (AZA0A8M6YZ52) performs well in IF, IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of ACC1/ACACA using anti-ACC1/ACACA antibody (AZA0A8M6YZ52).
ACC1/ACACA was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ACC1/ACACA Antibody (AZA0A8M6YZ52) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ACC1/ACACA was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ACC1/ACACA Antibody (AZA0A8M6YZ52) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Zebrafish ACC1/ACACA Antibody Picoband®
ACACA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IF, IHC, WB |
Tested Applications
ACACA antibody was positively detected by WB in:
zebrafish head tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ACACA antibody was positively detected by IHC in:
zebrafish liver tissue, zebrafish ovary tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ACACA antibody was positively detected by IF in:
zebrafish embryo tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
Immunofluorescence
2 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish ACC1/ACACA Antibody Picoband®
View all ACACA Antibodies
View all ACACA Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish ACC1/ACACA recombinant protein (Position: I799-H1324).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of ACACA
Predicted to enable acetyl-CoA carboxylase activity. Acts upstream of or within response to (R)-carnitine. Predicted to be located in cytoplasm. Predicted to be active in mitochondrion. Is expressed in female organism; liver; male organism; subcutaneous fat; and visceral fat. Orthologous to human ACACA (acetyl-CoA carboxylase alpha).
Cite This Product
Anti-Zebrafish ACC1/ACACA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A8M6YZ52)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Human
87%
Moderate Homology
Rabbit
87%
Moderate Homology
Dog
87%
Moderate Homology
Sheep
86%
Moderate Homology
Rat
86%
Moderate Homology
Cow
86%
Moderate Homology
Mouse
86%
Moderate Homology
Monkey
84%
Moderate Homology
Drosophila
53%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ACACA antibody
Immunohistochemistry (IHC) protocol for ACACA antibody
Immunofluorescence (IF) protocol for ACACA antibody
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Product Promise
We promise Anti-Zebrafish ACC1/ACACA Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZA0A8M6YZ52
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026