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Product Info Summary
| SKU: | AZA0A8M9PH45 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish CLOCKA Antibody Picoband®
SKU/Catalog Number
AZA0A8M9PH45
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish CLOCKA Antibody Picoband® catalog # AZA0A8M9PH45. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish CLOCKA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A8M9PH45)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish CLOCKA recombinant protein (Position: M1-Q562).
Reactive Species
AZA0A8M9PH45 is reactive to CLOCKA in Zebrafish
Background of CLOCKA
Enables DNA-binding transcription factor activity, RNA polymerase II-specific. Contributes to DNA binding activity. Involved in positive regulation of transcription by RNA polymerase II. Acts upstream of or within photoperiodism; regulation of gene expression; and response to temperature stimulus. Part of CLOCK-BMAL transcription complex. Is active in chromatin. Is expressed in brain; head; ovary; and photoreceptor cell. Human ortholog(s) of this gene implicated in Alzheimer's disease and alcohol use disorder. Orthologous to human CLOCK (clock circadian regulator).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA0A8M9PH45 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western Blot (WB) | 0.25-0.5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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Western blot analysis of CLOCKA using anti-CLOCKA antibody (AZA0A8M9PH45).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: whole female zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLOCKA antigen affinity purified polyclonal antibody (AZA0A8M9PH45) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CLOCKA at approximately 100 kDa. The expected band size for CLOCKA is at 100 kDa.
Specific Publications For Anti-Zebrafish CLOCKA Antibody Picoband® (AZA0A8M9PH45)
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