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Product Info Summary
| SKU: | AZQ3B7R0 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Zebrafish CYC1 Antibody Picoband®
SKU/Catalog Number
AZQ3B7R0
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish CYC1 Antibody Picoband® catalog # AZQ3B7R0. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish CYC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ3B7R0)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish CYC1 recombinant protein (Position: R93-K268).
Reactive Species
AZQ3B7R0 is reactive to CYC1 in Zebrafish
Observed Molecular Weight
35 kDa
Background of CYC1
Predicted to enable heme binding activity; metal ion binding activity; and ubiquinol-cytochrome-c reductase activity. Predicted to be involved in mitochondrial electron transport, ubiquinol to cytochrome c. Predicted to be located in mitochondrial inner membrane. Predicted to be part of respiratory chain complex III. Is expressed in several structures, including adaxial cell; digestive system; eye; midbrain; and musculature system. Human ortholog(s) of this gene implicated in mitochondrial complex III deficiency nuclear type 6. Orthologous to human CYC1 (cytochrome c1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ3B7R0 is guaranteed for IHC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Zebrafish |
| Immunohistochemistry (Paraffin-embedded Section) | 2-5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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Western blot analysis of CYC1 using anti-CYC1 antibody (AZQ3B7R0).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: whole female zebrafish tissue lysates,
Lane 3: whole male zebrafish tissue lysates,
Lane 4: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYC1 antigen affinity purified polyclonal antibody (AZQ3B7R0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CYC1 at approximately 35 kDa. The expected band size for CYC1 is at 35 kDa.
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IHC analysis of CYC1 using anti-CYC1 antibody (AZQ3B7R0).
CYC1 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYC1 Antibody (AZQ3B7R0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish CYC1 Antibody Picoband® (AZQ3B7R0)
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