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Product Info Summary
| SKU: | AZA0A8M6YV96 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish CYP11C1 Antibody
SKU/Catalog Number
AZA0A8M6YV96
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish CYP11C1 Antibody catalog # AZA0A8M6YV96. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish CYP11C1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A8M6YV96)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish CYP11C1 recombinant protein (Position: D23-H701).
Reactive Species
AZA0A8M6YV96 is reactive to CYP11C1 in Zebrafish
Background of CYP11C1
Predicted to enable steroid 11-beta-monooxygenase activity. Acts upstream of or within cortisol metabolic process; male gonad development; and mating behavior. Predicted to be located in mitochondrial membrane and synapse. Predicted to be active in mitochondrial inner membrane. Is expressed in Leydig cell; brain; and pleuroperitoneal region. Used to study congenital adrenal hyperplasia. Human ortholog(s) of this gene implicated in several diseases, including congenital adrenal hyperplasia; corticosterone methyloxidase deficiency 1; diabetes mellitus (multiple); hypertension (multiple); and primary hyperaldosteronism (multiple). Orthologous to several human genes including CYP11B1 (cytochrome P450 family 11 subfamily B member 1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA0A8M6YV96 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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IHC analysis of CYP11C1 using anti-CYP11C1 antibody (AZA0A8M6YV96).
CYP11C1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP11C1 Antibody (AZA0A8M6YV96) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish CYP11C1 Antibody (AZA0A8M6YV96)
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