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Product Info Summary
| SKU: | AZQ7T346 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish DDIT4 Antibody Picoband®
SKU/Catalog Number
AZQ7T346
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish DDIT4 Antibody Picoband® catalog # AZQ7T346. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish DDIT4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ7T346)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish DDIT4 recombinant protein (Position: M1-C220).
Reactive Species
AZQ7T346 is reactive to DDIT4 in Zebrafish
Background of DDIT4
Predicted to enable 14-3-3 protein binding activity. Acts upstream of or within dorsal/ventral pattern formation. Predicted to be located in cytoplasm. Is expressed in several structures, including central nervous system; digestive system; mesoderm; muscle; and pleuroperitoneal region. Orthologous to human DDIT4 (DNA damage inducible transcript 4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ7T346 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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Western blot analysis of DDIT4 using anti-DDIT4 antibody (AZQ7T346).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDIT4 antigen affinity purified polyclonal antibody (AZQ7T346) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDIT4 at approximately 24 kDa. The expected band size for DDIT4 is at 24 kDa.
Specific Publications For Anti-Zebrafish DDIT4 Antibody Picoband® (AZQ7T346)
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