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DRD4A antibody (AZA0A0R4IR92) performs well in WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of DRD4A using anti-DRD4A antibody (AZA0A0R4IR92).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: whole female zebrafish tissue lysates,
Lane 3: whole male zebrafish tissue lysates,
Lane 4: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRD4A antigen affinity purified polyclonal antibody (AZA0A0R4IR92) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DRD4A at approximately 43 kDa. The expected band size for DRD4A is at 43 kDa.
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: whole female zebrafish tissue lysates,
Lane 3: whole male zebrafish tissue lysates,
Lane 4: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRD4A antigen affinity purified polyclonal antibody (AZA0A0R4IR92) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DRD4A at approximately 43 kDa. The expected band size for DRD4A is at 43 kDa.
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Anti-Zebrafish DRD4A Antibody Picoband®
DRD4A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | WB |
Tested Applications
DRD4A antibody was positively detected by WB in:
zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish DRD4A Antibody Picoband®
View all DRD4A Antibodies
View all DRD4A Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish DRD4A recombinant protein (Position: M1-R300).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of DRD4A
Predicted to enable G protein-coupled receptor activity and dopamine neurotransmitter receptor activity, coupled via Gi/Go. Acts upstream of or within spinal cord motor neuron cell fate specification and ventral spinal cord interneuron differentiation. Predicted to be located in membrane. Predicted to be active in plasma membrane. Is expressed in nervous system. Human ortholog(s) of this gene implicated in several diseases, including Gilles de la Tourette syndrome; antisocial personality disorder; attention deficit hyperactivity disorder; conduct disorder; and substance abuse (multiple). Orthologous to human DRD4 (dopamine receptor D4).
Cite This Product
Anti-Zebrafish DRD4A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A0R4IR92)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Human
53%
Low homology
Rat
52%
Low homology
Mouse
51%
Low homology
Drosophila
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for DRD4A antibody
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Product Promise
We promise Anti-Zebrafish DRD4A Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZA0A0R4IR92
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026