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FOXJ1A antibody (AZF1QDF8) performs well in IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of FOXJ1A using anti-FOXJ1A antibody (AZF1QDF8).
FOXJ1A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXJ1A Antibody (AZF1QDF8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
FOXJ1A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXJ1A Antibody (AZF1QDF8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish FOXJ1A Antibody
FOXJ1A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC |
Tested Applications
FOXJ1A antibody was positively detected by IHC in:
zebrafish brain tissue, zebrafish spinal cord tissue, zebrafish kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish FOXJ1A Antibody
View all FOXJ1A Antibodies
View all FOXJ1A Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish FOXJ1A recombinant protein (Position: K217-S338).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of FOXJ1A
Enables sequence-specific DNA binding activity. Acts upstream of or within several processes, including central canal development; cerebrospinal fluid circulation; and determination of heart left/right asymmetry. Predicted to be active in nucleus. Is expressed in several structures, including embryonic structure; mesoderm; nervous system; neural tube; and renal system. Human ortholog(s) of this gene implicated in primary ciliary dyskinesia 43. Orthologous to human FOXJ1 (forkhead box J1).
Cite This Product
Anti-Zebrafish FOXJ1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZF1QDF8)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Human
48%
Low homology
Rabbit
48%
Low homology
Monkey
48%
Low homology
Cow
46%
Low homology
Sheep
46%
Low homology
Rat
44%
Low homology
Mouse
43%
Low homology
Dog
43%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for FOXJ1A antibody
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Product Promise
We promise Anti-Zebrafish FOXJ1A Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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AZF1QDF8
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026