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Product Info Summary
| SKU: | AZB3DIG4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish FZD5 Antibody Picoband®
SKU/Catalog Number
AZB3DIG4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish FZD5 Antibody Picoband® catalog # AZB3DIG4. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish FZD5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZB3DIG4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish FZD5 recombinant protein (Position: M1-V592).
Reactive Species
AZB3DIG4 is reactive to FZD5 in Zebrafish
Observed Molecular Weight
66 kDa
Background of FZD5
Enables Wnt receptor activity. Involved in eye development and non-canonical Wnt signaling pathway. Acts upstream of or within several processes, including liver development; negative regulation of canonical Wnt signaling pathway; and swim bladder development. Predicted to be located in several cellular components, including Golgi membrane; dendrite; and perikaryon. Predicted to be active in plasma membrane. Is expressed in several structures, including endoderm; eye; gut; hematopoietic stem cell; and neural plate. Used to study coloboma. Orthologous to human FZD5 (frizzled class receptor 5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZB3DIG4 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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Western blot analysis of FZD5 using anti-FZD5 antibody (AZB3DIG4).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FZD5 antigen affinity purified polyclonal antibody (AZB3DIG4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FZD5 at approximately 66 kDa. The expected band size for FZD5 is at 66 kDa.
Specific Publications For Anti-Zebrafish FZD5 Antibody Picoband® (AZB3DIG4)
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