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Product Info Summary
| SKU: | AZQ9YI00 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish FZD8A Antibody
SKU/Catalog Number
AZQ9YI00
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish FZD8A Antibody catalog # AZQ9YI00. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish FZD8A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ9YI00)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish FZD8A recombinant protein (Position: P18-V579).
Reactive Species
AZQ9YI00 is reactive to FZD8A in Zebrafish
Background of FZD8A
Predicted to enable Wnt receptor activity and Wnt-protein binding activity. Acts upstream of or within canonical Wnt signaling pathway; eye field cell fate commitment involved in camera-type eye formation; and neurogenesis. Predicted to be located in membrane. Predicted to be active in plasma membrane. Is expressed in several structures, including digestive system; mesoderm; nervous system; neural keel; and pectoral fin. Orthologous to human FZD8 (frizzled class receptor 8).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ9YI00 is guaranteed for IHC Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Immunohistochemistry | 2-5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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IHC analysis of FZD8A using anti-FZD8A antibody (AZQ9YI00).
FZD8A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FZD8A Antibody (AZQ9YI00) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish FZD8A Antibody (AZQ9YI00)
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