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GFRA3 antibody (AZE7F4G1) performs well in IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of GFRA3 using anti-GFRA3 antibody (AZE7F4G1).
GFRA3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFRA3 Antibody (AZE7F4G1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
GFRA3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFRA3 Antibody (AZE7F4G1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish GFRA3 Antibody Picoband®
GFRA3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC, WB |
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry (Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish GFRA3 Antibody Picoband®
View all GFRA3 Antibodies
View all GFRA3 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish GFRA3 recombinant protein (Position: E32-E205).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
51 kDa
Background of GFRA3
Predicted to enable signaling receptor activity. Acts upstream of or within branchiomeric skeletal muscle development. Predicted to be located in plasma membrane. Predicted to be part of receptor complex. Predicted to be active in external side of plasma membrane. Is expressed in hyoid muscle; pharyngeal arch; pharyngeal arch 1; pharyngeal arch 2; and pharyngeal musculature. Orthologous to human GFRA3 (GDNF family receptor alpha 3).
Cite This Product
Anti-Zebrafish GFRA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZE7F4G1)
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Product Promise
We promise Anti-Zebrafish GFRA3 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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AZE7F4G1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Special Price
$99.00
Regular Price
$470.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026