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Product Info Summary
| SKU: | AZQ1XHK0 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish Glucocorticoid receptor/NR3C1 Antibody Picoband®
SKU/Catalog Number
AZQ1XHK0
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish Glucocorticoid receptor/NR3C1 Antibody Picoband® catalog #AZQ1XHK0. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish Glucocorticoid receptor/NR3C1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ1XHK0)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish Glucocorticoid receptor/NR3C1 recombinant protein (Position: M1-K746)
Reactive Species
AZQ1XHK0 is reactive to NR3C1 in Zebrafish
Observed Molecular Weight
94 kDa
Background of NR3C1
The glucocorticoid receptor (GR, or GCR), also known as NR3C1, is the receptor to which cortisol and other glucocorticoids bind. In humans, the GR protein is encoded by NR3C1 gene which is located on chromosome 5 (5q31). GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response. Because the receptor gene is expressed in several forms, it has many different (pleiotropic) effects in different parts of the body. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or represses the expression of pro-inflammatory proteins in the cytosol (by preventing the translocation of other transcription factors from the cytosol into the nucleus).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ1XHK0 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Positive Control
WB: zebrafish head tissue
Validation Images & Assay Conditions
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Western blot analysis of Glucocorticoid receptor/NR3C1 using anti-Glucocorticoid receptor/NR3C1 antibody (AZQ1XHK0).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glucocorticoid receptor/NR3C1 antigen affinity purified polyclonal antibody (AZQ1XHK0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Glucocorticoid receptor/NR3C1 at approximately 94 kDa. The expected band size for Glucocorticoid receptor/NR3C1 is at 82 kDa.
Specific Publications For Anti-Zebrafish Glucocorticoid receptor/NR3C1 Antibody Picoband® (AZQ1XHK0)
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