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NR3C1 antibody (AZQ1XHK0) performs well in WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of Glucocorticoid receptor/NR3C1 using anti-Glucocorticoid receptor/NR3C1 antibody (AZQ1XHK0).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glucocorticoid receptor/NR3C1 antigen affinity purified polyclonal antibody (AZQ1XHK0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Glucocorticoid receptor/NR3C1 at approximately 94 kDa. The expected band size for Glucocorticoid receptor/NR3C1 is at 82 kDa.
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glucocorticoid receptor/NR3C1 antigen affinity purified polyclonal antibody (AZQ1XHK0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Glucocorticoid receptor/NR3C1 at approximately 94 kDa. The expected band size for Glucocorticoid receptor/NR3C1 is at 82 kDa.
Designing a Western blot for NR3C1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the NR3C1 Western Blot GuideBundle Save
Anti-Zebrafish Glucocorticoid receptor/NR3C1 Antibody Picoband®
NR3C1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | WB |
Tested Applications
NR3C1 antibody was positively detected by WB in:
zebrafish head tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish Glucocorticoid receptor/NR3C1 Antibody Picoband®
View all NR3C1 Antibodies
View all NR3C1 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish Glucocorticoid receptor/NR3C1 recombinant protein (Position: M1-K746)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
94 kDa
Background of NR3C1
The glucocorticoid receptor (GR, or GCR), also known as NR3C1, is the receptor to which cortisol and other glucocorticoids bind. In humans, the GR protein is encoded by NR3C1 gene which is located on chromosome 5 (5q31). GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response. Because the receptor gene is expressed in several forms, it has many different (pleiotropic) effects in different parts of the body. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or represses the expression of pro-inflammatory proteins in the cytosol (by preventing the translocation of other transcription factors from the cytosol into the nucleus).
Cite This Product
Anti-Zebrafish Glucocorticoid receptor/NR3C1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ1XHK0)
Protocols
Western Blot protocol for NR3C1 antibody
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Product Promise
We promise Anti-Zebrafish Glucocorticoid receptor/NR3C1 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZQ1XHK0
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 22, 2026