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Product Info Summary
| SKU: | AZQ5Y835 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish GNRH2 Antibody
SKU/Catalog Number
AZQ5Y835
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish GNRH2 Antibody catalog # AZQ5Y835. Tested in IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish GNRH2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ5Y835)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish GNRH2 recombinant protein (Position: Q25-K86)
Reactive Species
AZQ5Y835 is reactive to GNRH2 in Zebrafish
Background of GNRH2
Predicted to enable gonadotropin hormone-releasing hormone activity and gonadotropin-releasing hormone receptor binding activity. Acts upstream of or within brain development; camera-type eye development; and regulation of feeding behavior. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including brain; female organism; gonad; male organism; and musculature system. Orthologous to human GNRH2 (gonadotropin releasing hormone 2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ5Y835 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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IHC analysis of GNRH2 using anti-GNRH2 antibody (AZQ5Y835).
GNRH2 was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNRH2 Antibody (AZQ5Y835) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of GNRH2 using anti-GNRH2 antibody (AZQ5Y835).
GNRH2 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNRH2 Antibody (AZQ5Y835) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of GNRH2 using anti-GNRH2 antibody (AZQ5Y835).
GNRH2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNRH2 Antibody (AZQ5Y835) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish GNRH2 Antibody (AZQ5Y835)
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