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GNSa antibody (AZQ4V902) performs well in IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of GNSa using anti-GNSa antibody (AZQ4V902).
GNSa was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNSa Antibody (AZQ4V902) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
GNSa was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNSa Antibody (AZQ4V902) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish GNSa Antibody Picoband®
GNSa antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC, WB |
Tested Applications
GNSa antibody was positively detected by WB in:
whole female zebrafish tissue, whole male zebrafish tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GNSa antibody was positively detected by IHC in:
zebrafish brain tissue, zebrafish colon tissue, zebrafish pancreas tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish GNSa Antibody Picoband®
View all GNSA Antibodies
View all GNSA Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish GNSa recombinant protein (Position: W224-R341).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
95 kDa
Background of GNSa
N-acetylglucosamine-6-sulfatase, also known as glucosamine (N-acetyl)-6-sulfatase, is an enzyme that in humans is encoded by the GNS gene. The product of this gene is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin, heparan sulphate, and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder mucopolysaccharidosis type IIID (Sanfilippo D syndrome). Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome.
Cite This Product
Anti-Zebrafish GNSa Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ4V902)
Protocols
Western Blot protocol for GNSa antibody
Immunohistochemistry (IHC) protocol for GNSa antibody
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Product Promise
We promise Anti-Zebrafish GNSa Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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AZQ4V902
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 24, 2026