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GPHNa/b antibody (AZD3KYK7) performs well in IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of GPHNa/b using anti-GPHNa/b antibody (AZD3KYK7).
GPHNa/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPHNa/b Antibody (AZD3KYK7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
GPHNa/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPHNa/b Antibody (AZD3KYK7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish GPHNa/b Antibody Picoband®
GPHNa/b antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC, WB |
Tested Applications
GPHNa/b antibody was positively detected by WB in:
zebrafish head tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GPHNa/b antibody was positively detected by IHC in:
zebrafish brain tissue, zebrafish pancreas tissue, zebrafish colon tissue, zebrafish spinal cord tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish GPHNa/b Antibody Picoband®
View all GPHNA/B Antibodies
View all GPHNA/B Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish GPHNa/b recombinant protein (Position: V288-L735).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
97 kDa
Background of GPHNa/b
Gephyrin is a protein that in humans is encoded by the GPHN gene. This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known.
Cite This Product
Anti-Zebrafish GPHNa/b Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZD3KYK7)
Protocols
Western Blot protocol for GPHNa/b antibody
Immunohistochemistry (IHC) protocol for GPHNa/b antibody
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We promise Anti-Zebrafish GPHNa/b Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZD3KYK7
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 21, 2026