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Product Info Summary
| SKU: | AZU3N5X6 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish GRM2A Antibody Picoband®
SKU/Catalog Number
AZU3N5X6
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish GRM2A Antibody Picoband® catalog # AZU3N5X6. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish GRM2A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZU3N5X6)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish GRM2A recombinant protein (Position: D242-K489).
Reactive Species
AZU3N5X6 is reactive to GRM2A in Zebrafish
Observed Molecular Weight
100 kDa
Background of GRM2A
Predicted to enable group II metabotropic glutamate receptor activity. Predicted to be involved in G protein-coupled glutamate receptor signaling pathway and regulation of glutamatergic synaptic transmission. Predicted to act upstream of or within G protein-coupled receptor signaling pathway. Predicted to be located in membrane. Predicted to be active in plasma membrane. Is expressed in brain; retinal neural layer; and trigeminal ganglion. Orthologous to human GRM2 (glutamate metabotropic receptor 2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZU3N5X6 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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Western blot analysis of GRM2A using anti-GRM2A antibody (AZU3N5X6).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: whole female zebrafish tissue lysates,
Lane 2: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRM2A antigen affinity purified polyclonal antibody (AZU3N5X6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRM2A at approximately 100 kDa. The expected band size for GRM2A is at 100 kDa.
Specific Publications For Anti-Zebrafish GRM2A Antibody Picoband® (AZU3N5X6)
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