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Product Info Summary
| SKU: | AZA0A0R4IRN2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish GRM6A Antibody Picoband®
SKU/Catalog Number
AZA0A0R4IRN2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish GRM6A Antibody Picoband® catalog # AZA0A0R4IRN2. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish GRM6A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A0R4IRN2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish GRM6A recombinant protein (Position: N290-Q908).
Reactive Species
AZA0A0R4IRN2 is reactive to GRM6A in Zebrafish
Observed Molecular Weight
102 kDa
Background of GRM6A
Predicted to enable group III metabotropic glutamate receptor activity. Predicted to be involved in G protein-coupled glutamate receptor signaling pathway and regulation of glutamatergic synaptic transmission. Predicted to act upstream of or within G protein-coupled receptor signaling pathway. Predicted to be located in membrane. Predicted to be active in plasma membrane. Is expressed in brain and retinal neural layer. Human ortholog(s) of this gene implicated in congenital stationary night blindness 1B. Orthologous to human GRM6 (glutamate metabotropic receptor 6).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA0A0R4IRN2 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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Western blot analysis of GRM6A using anti-GRM6A antibody (AZA0A0R4IRN2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRM6A antigen affinity purified polyclonal antibody (AZA0A0R4IRN2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRM6A at approximately 102 kDa. The expected band size for GRM6A is at 102 kDa.
Specific Publications For Anti-Zebrafish GRM6A Antibody Picoband® (AZA0A0R4IRN2)
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