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Product Info Summary
| SKU: | AZE7F9U2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish GSDMEA Antibody
SKU/Catalog Number
AZE7F9U2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish GSDMEA Antibody catalog # AZE7F9U2. Tested in IHC applications.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish GSDMEA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZE7F9U2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish GSDMEA recombinant protein (Position: Q47-T280).
Reactive Species
AZE7F9U2 is reactive to GSDMEA in Zebrafish
Background of GSDMEA
Acts upstream of or within with a positive effect on pyroptotic inflammatory response. Predicted to be located in endomembrane system. Predicted to be active in cytoplasm. Is expressed in several structures, including gill; heart; inner ear; neuromast; and pleuroperitoneal region. Human ortholog(s) of this gene implicated in autosomal dominant nonsyndromic deafness 5 and sensorineural hearing loss. Orthologous to human GSDME (gasdermin E).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZE7F9U2 is guaranteed for IHC Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Immunohistochemistry (Paraffin-embedded Section) | 2-5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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IHC analysis of GSDMEA using anti-GSDMEA antibody (AZE7F9U2).
GSDMEA was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSDMEA Antibody (AZE7F9U2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of GSDMEA using anti-GSDMEA antibody (AZE7F9U2).
GSDMEA was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSDMEA Antibody (AZE7F9U2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of GSDMEA using anti-GSDMEA antibody (AZE7F9U2).
GSDMEA was detected in a paraffin-embedded section of zebrafish intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSDMEA Antibody (AZE7F9U2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish GSDMEA Antibody (AZE7F9U2)
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