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Product Info Summary
| SKU: | AZQ90466 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish HER4(1+2+3+5) Antibody
View all HER4(1+2+3+5) Antibodies
SKU/Catalog Number
AZQ90466
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish HER4(1+2+3+5) Antibody catalog # AZQ90466. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish HER4(1+2+3+5) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ90466)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish HER4(1+2+3+5) recombinant protein (Position: M1-W152)
Reactive Species
AZQ90466 is reactive to HER4(1+2+3+5) in Zebrafish
Background of HER4(1+2+3+5)
Enables double-stranded DNA binding activity. Acts upstream of or within several processes, including Notch signaling pathway; nervous system development; and somite specification. Predicted to be active in nucleus. Is expressed in several structures, including anterior neural rod; embryonic structure; nervous system; neural keel; and segmental plate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ90466 is guaranteed for IHC Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Immunohistochemistry (IHC) | 2-5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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IHC analysis of HER4(1+2+3+5) using anti-HER4(1+2+3+5) antibody (AZQ90466).
HER4(1+2+3+5) was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HER4(1+2+3+5) Antibody (AZQ90466) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of HER4(1+2+3+5) using anti-HER4(1+2+3+5) antibody (AZQ90466).
HER4(1+2+3+5) was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HER4(1+2+3+5) Antibody (AZQ90466) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish HER4(1+2+3+5) Antibody (AZQ90466)
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