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HER4(1+2+3+5) antibody (AZQ90466) performs well in IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of HER4(1+2+3+5) using anti-HER4(1+2+3+5) antibody (AZQ90466).
HER4(1+2+3+5) was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HER4(1+2+3+5) Antibody (AZQ90466) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
HER4(1+2+3+5) was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HER4(1+2+3+5) Antibody (AZQ90466) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish HER4(1+2+3+5) Antibody
HER4(1+2+3+5) antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC |
Tested Applications
HER4(1+2+3+5) antibody was positively detected by IHC in:
zebrafish brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish HER4(1+2+3+5) Antibody
View all HER4(1+2+3+5) Antibodies
View all HER4(1+2+3+5) Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish HER4(1+2+3+5) recombinant protein (Position: M1-W152)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of HER4(1+2+3+5)
Enables double-stranded DNA binding activity. Acts upstream of or within several processes, including Notch signaling pathway; nervous system development; and somite specification. Predicted to be active in nucleus. Is expressed in several structures, including anterior neural rod; embryonic structure; nervous system; neural keel; and segmental plate.
Cite This Product
Anti-Zebrafish HER4(1+2+3+5) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ90466)
Protocols
Immunohistochemistry (IHC) protocol for HER4(1+2+3+5) antibody
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Product Promise
We promise Anti-Zebrafish HER4(1+2+3+5) Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
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AZQ90466
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026