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- Table of Contents
IGF1RA antibody (AZA0A8M6YTF7) performs well in IF, IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of IGF1RA using anti-IGF1RA antibody (AZA0A8M6YTF7).
IGF1RA was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-IGF1RA Antibody (AZA0A8M6YTF7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IGF1RA was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-IGF1RA Antibody (AZA0A8M6YTF7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Zebrafish IGF1RA Antibody
IGF1RA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IF, IHC |
Tested Applications
IGF1RA antibody was positively detected by IHC in:
zebrafish colon tissue, zebrafish muscle tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
IGF1RA antibody was positively detected by IF in:
zebrafish embryo tissue
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
Immunofluorescence
2 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish IGF1RA Antibody
View all IGF1RA Antibodies
View all IGF1RA Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish IGF1RA recombinant protein (Position: H36-R886).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of IGF1RA
Enables cysteine-type endopeptidase inhibitor activity involved in apoptotic process and insulin-like growth factor binding activity. Acts upstream of or within several processes, including embryonic organ development; fin regeneration; and insulin-like growth factor receptor signaling pathway. Predicted to be located in membrane. Predicted to be part of insulin receptor complex. Predicted to be active in axon and plasma membrane. Is expressed in several structures, including digestive system; female organism; male organism; musculature system; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including IgA glomerulonephritis; dementia (multiple); kidney cancer (multiple); liver disease (multiple); and neurodegenerative disease (multiple). Orthologous to human IGF1R (insulin like growth factor 1 receptor).
Cite This Product
Anti-Zebrafish IGF1RA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A8M6YTF7)
Protocols
Immunohistochemistry (IHC) protocol for IGF1RA antibody
Immunofluorescence (IF) protocol for IGF1RA antibody
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Product Promise
We promise Anti-Zebrafish IGF1RA Antibody performs excellently in relevant applications for IF, IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
AZA0A8M6YTF7
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026