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Product Info Summary
| SKU: | AZC4MCP0 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish IGFBP6A Antibody Picoband®
SKU/Catalog Number
AZC4MCP0
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish IGFBP6A Antibody Picoband® catalog # AZC4MCP0. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish IGFBP6A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZC4MCP0)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish IGFBP6A recombinant protein (Position: D46-K175).
Reactive Species
AZC4MCP0 is reactive to IGFBP6A in Zebrafish
Background of IGFBP6A
Predicted to enable fibronectin binding activity; insulin-like growth factor I binding activity; and insulin-like growth factor II binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including eye; gill; heart; liver; and pleuroperitoneal region. Human ortholog(s) of this gene implicated in obesity. Orthologous to human IGFBP6 (insulin like growth factor binding protein 6).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZC4MCP0 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Zebrafish |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of IGFBP6A using anti-IGFBP6A antibody (AZC4MCP0).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: whole female zebrafish tissue lysates,
Lane 3: whole male zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP6A antigen affinity purified polyclonal antibody (AZC4MCP0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IGFBP6A at approximately 25 kDa. The expected band size for IGFBP6A is at 21 kDa.
Specific Publications For Anti-Zebrafish IGFBP6A Antibody Picoband® (AZC4MCP0)
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