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Product Info Summary
| SKU: | AZA3KNS3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Zebrafish IHHA Antibody Picoband®
SKU/Catalog Number
AZA3KNS3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish IHHA Antibody Picoband® catalog # AZA3KNS3. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish IHHA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA3KNS3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish IHHA recombinant protein (Position: A203-R413).
Reactive Species
AZA3KNS3 is reactive to IHHA in Zebrafish
Background of IHHA
Predicted to enable calcium ion binding activity and patched binding activity. Acts upstream of or within several processes, including enteric nervous system development; intramembranous ossification; and swim bladder morphogenesis. Predicted to be located in several cellular components, including Golgi membrane; endoplasmic reticulum membrane; and extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including caudal fin skeleton; cranium; digestive system; pneumatic duct; and swim bladder bud. Human ortholog(s) of this gene implicated in acrocapitofemoral dysplasia; brachydactyly type A1; retinopathy of prematurity; and syndactyly type 1. Orthologous to human IHH (Indian hedgehog signaling molecule).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA3KNS3 is guaranteed for IHC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Zebrafish |
| Immunohistochemistry(Paraffin-embedded Section) | 2-5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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Western blot analysis of IHHA using anti-IHHA antibody (AZA3KNS3).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IHHA antigen affinity purified polyclonal antibody (AZA3KNS3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IHHA at approximately 45 kDa. The expected band size for IHHA is at 45 kDa.
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IHC analysis of IHHA using anti-IHHA antibody (AZA3KNS3).
IHHA was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IHHA Antibody (AZA3KNS3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish IHHA Antibody Picoband® (AZA3KNS3)
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