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Product Info Summary
| SKU: | AZA0A0R4IMZ8 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish Importin Beta/KPNB1 Antibody Picoband®
SKU/Catalog Number
AZA0A0R4IMZ8
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish Importin Beta/KPNB1 Antibody Picoband® catalog #AZA0A0R4IMZ8. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish Importin Beta/KPNB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A0R4IMZ8)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish Importin Beta/KPNB1 recombinant protein (Position: E8-K871)
Reactive Species
AZA0A0R4IMZ8 is reactive to KPNB1 in Zebrafish
Observed Molecular Weight
97 kDa
Background of KPNB1
Importin subunit beta-1 is a protein that in humans is encoded by the KPNB1 gene. Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA0A0R4IMZ8 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Positive Control
WB: zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue
Validation Images & Assay Conditions
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Western blot analysis of Importin Beta/KPNB1 using anti-Importin Beta/KPNB1 antibody (AZA0A0R4IMZ8).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: whole female zebrafish tissue lysates,
Lane 3: whole male zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Importin Beta/KPNB1 antigen affinity purified polyclonal antibody (AZA0A0R4IMZ8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Importin Beta/KPNB1 at approximately 97 kDa. The expected band size for Importin Beta/KPNB1 is at 97 kDa.
Specific Publications For Anti-Zebrafish Importin Beta/KPNB1 Antibody Picoband® (AZA0A0R4IMZ8)
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