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NEDD8/8l antibody (AZF1QMF9) performs well in IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of NEDD8/8l using anti-NEDD8/8l antibody (AZF1QMF9).
NEDD8/8l was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEDD8/8l Antibody (AZF1QMF9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
NEDD8/8l was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEDD8/8l Antibody (AZF1QMF9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish NEDD8/8l Antibody
NEDD8/8l antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC |
Tested Applications
NEDD8/8l antibody was positively detected by IHC in:
zebrafish brain tissue, zebrafish spinal cord tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Zebrafish
Tested
Recommend
Human
Predicted
Predicted
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Rabbit
Predicted
Predicted
Bovine
Predicted
Predicted
Dog
Predicted
Predicted
Fruit fly
Predicted
Predicted
Product Information
Product Name
Anti-Zebrafish NEDD8/8l Antibody
View all NEDD8/8L Antibodies
View all NEDD8/8L Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish NEDD8/8l recombinant protein (Position: M1-G76)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of NEDD8/8l
NEDD8 is a protein that in humans is encoded by the NEDD8 gene. Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The most substrates of NEDD8 modification are the Cullin subunits of Cullin-based E3 ubiquitin ligases, which are active only when neddylated. Their NEDDylation is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.
Cite This Product
Anti-Zebrafish NEDD8/8l Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZF1QMF9)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Human
99%
High Homology
Mouse
99%
High Homology
Rat
99%
High Homology
Rabbit
99%
High Homology
Cow
99%
High Homology
Dog
99%
High Homology
Drosophila
95%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for NEDD8/8l antibody
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Product Promise
We promise Anti-Zebrafish NEDD8/8l Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
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AZF1QMF9
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026