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NFKBIAA antibody (AZF1QCG4) performs well in IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of NFKBIAA using anti-NFKBIAA antibody (AZF1QCG4).
NFKBIAA was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NFKBIAA Antibody (AZF1QCG4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
NFKBIAA was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NFKBIAA Antibody (AZF1QCG4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish NFKBIAA Antibody Picoband®
NFKBIAA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC, WB |
Tested Applications
NFKBIAA antibody was positively detected by WB in:
zebrafish head tissue, whole female zebrafish tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NFKBIAA antibody was positively detected by IHC in:
zebrafish kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish NFKBIAA Antibody Picoband®
View all NFKBIAA Antibodies
View all NFKBIAA Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish NFKBIAA recombinant protein (Position: M1-V227).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of NFKBIAA
Predicted to enable NF-kappaB binding activity and protein sequestering activity. Acts upstream of or within negative regulation of hematopoietic progenitor cell differentiation and response to virus. Predicted to be active in cytosol. Is expressed in several structures, including lateral line system; periderm; pronephric duct; tail bud; and vent. Human ortholog(s) of this gene implicated in several diseases, including childhood-onset asthma; ectodermal dysplasia and immunodeficiency 2; hematologic cancer (multiple); hepatitis B; and lung disease (multiple). Orthologous to human NFKBIA (NFKB inhibitor alpha).
Cite This Product
Anti-Zebrafish NFKBIAA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZF1QCG4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Rabbit
52%
Low homology
Dog
51%
Low homology
Mouse
51%
Low homology
Cow
51%
Low homology
Human
50%
Low homology
Rat
50%
Low homology
Sheep
50%
Low homology
Monkey
50%
Low homology
Drosophila
34%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NFKBIAA antibody
Immunohistochemistry (IHC) protocol for NFKBIAA antibody
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Product Promise
We promise Anti-Zebrafish NFKBIAA Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZF1QCG4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026