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Product Info Summary
| SKU: | AZD9MNA7 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish PDGFRB Antibody Picoband®
SKU/Catalog Number
AZD9MNA7
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish PDGFRB Antibody Picoband® catalog # AZD9MNA7. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish PDGFRB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZD9MNA7)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish PDGFRB recombinant protein (Position: N103-R493).
Reactive Species
AZD9MNA7 is reactive to PDGFRB in Zebrafish
Background of PDGFRB
Predicted to enable platelet-derived growth factor beta-receptor activity and platelet-derived growth factor binding activity. Involved in several processes, including angiogenesis involved in coronary vascular morphogenesis; platelet-derived growth factor receptor-beta signaling pathway; and positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction. Acts upstream of or within several processes, including embryonic neurocranium morphogenesis; hematopoietic stem cell differentiation; and vasculature development. Predicted to be located in membrane. Predicted to be part of receptor complex. Predicted to be active in plasma membrane. Is expressed in several structures, including digestive system; floor plate neural tube; hypochord; somite; and vasculature. Human ortholog(s) of this gene implicated in basal ganglia calcification; glioblastoma; infantile myofibromatosis; myeloproliferative neoplasm; and renal cell carcinoma. Orthologous to human PDGFRB (platelet derived growth factor receptor beta).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZD9MNA7 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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Western blot analysis of PDGFRB using anti-PDGFRB antibody (AZD9MNA7).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRB antigen affinity purified polyclonal antibody (AZD9MNA7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDGFRB at approximately 120 kDa. The expected band size for PDGFRB is at 120 kDa.
Specific Publications For Anti-Zebrafish PDGFRB Antibody Picoband® (AZD9MNA7)
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