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PHYHIPL/b antibody (AZA4QNW7) performs well in IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of PHYHIPL/b using anti-PHYHIPL/b antibody (AZA4QNW7).
PHYHIPL/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PHYHIPL/b Antibody (AZA4QNW7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
PHYHIPL/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PHYHIPL/b Antibody (AZA4QNW7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish PHYHIPL/b Antibody
PHYHIPL/b antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC |
Tested Applications
PHYHIPL/b antibody was positively detected by IHC in:
zebrafish brain tissue, zebrafish spinal tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish PHYHIPL/b Antibody
View all PHYHIPL/B Antibodies
View all PHYHIPL/B Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish PHYHIPL/b recombinant protein (Position: M1-D337).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of PHYHIPL/b
Phytanoyl-CoA hydroxylase-interacting protein-like is an enzyme that in humans is encoded by the PHYHIPL gene. PHYHIPL (phytanoyl-CoA 2-hydroxylase interacting protein-like), also known as phytanoyl-CoA hydroxylase-interacting protein-like, is a 376 amino acid protein that contains one fibronectin type-III domain and belongs to the PHYHIP family. Conserved in chimpanzee, canine, mouse, rat, chicken, zebrafish and Caenorhabditis elegans, PHYHIPL exists as three alternatively spliced isoforms. PHYHIPL is a down-regulated target of IRX1, a homeobox tumor suppressor gene linked to gastric carcinoma. PHYHIPL may also play a role in the development of the central system. The gene that encodes PHYHIPL maps to human chromosome 10q21.1.
Cite This Product
Anti-Zebrafish PHYHIPL/b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA4QNW7)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Human
84%
Moderate Homology
Cow
84%
Moderate Homology
Monkey
84%
Moderate Homology
Mouse
84%
Moderate Homology
Dog
84%
Moderate Homology
Rat
83%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for PHYHIPL/b antibody
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Product Promise
We promise Anti-Zebrafish PHYHIPL/b Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
AZA4QNW7
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026