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PPP3R1a/b antibody (AZA0A8M3BEF6) performs well in IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of PPP3R1a/b using anti-PPP3R1a/b antibody (AZA0A8M3BEF6).
PPP3R1a/b was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP3R1a/b Antibody (AZA0A8M3BEF6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
PPP3R1a/b was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP3R1a/b Antibody (AZA0A8M3BEF6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish PPP3R1a/b Antibody Picoband®
PPP3R1a/b antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC, WB |
Tested Applications
PPP3R1a/b antibody was positively detected by WB in:
zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PPP3R1a/b antibody was positively detected by IHC in:
zebrafish heart tissue, zebrafish brain tissue, zebrafish spinal tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Zebrafish
Tested
Tested
Recommend
Human
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Zebrafish PPP3R1a/b Antibody Picoband®
View all PPP3R1A/B Antibodies
View all PPP3R1A/B Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish PPP3R1a/b recombinant protein (Position: E53-V175).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of PPP3R1a/b
Calcineurin, the Ca(2+)/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in all cells from yeast to mammals. Calcineurin A alpha(PPP3CA),is located on human chromosomes 4, Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase(calcineurin) catalytic subunit. Calcineurin regulates bone formation by the osteoblast.
Cite This Product
Anti-Zebrafish PPP3R1a/b Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A8M3BEF6)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Human
99%
High Homology
Mouse
99%
High Homology
Rat
99%
High Homology
Cow
99%
High Homology
Drosophila
89%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PPP3R1a/b antibody
Immunohistochemistry (IHC) protocol for PPP3R1a/b antibody
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Product Promise
We promise Anti-Zebrafish PPP3R1a/b Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
AZA0A8M3BEF6
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026