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Product Info Summary
| SKU: | AZQ7ZSX3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish PTF1A Antibody
SKU/Catalog Number
AZQ7ZSX3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish PTF1A Antibody catalog # AZQ7ZSX3. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish PTF1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ7ZSX3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish PTF1A recombinant protein (Position: D27-S265).
Reactive Species
AZQ7ZSX3 is reactive to PTF1A in Zebrafish
Background of PTF1A
Enables DNA-binding transcription factor activity and sequence-specific DNA binding activity. Acts upstream of or within several processes, including exocrine pancreas development; neuron differentiation; and positive regulation of DNA-templated transcription. Predicted to be located in cytoplasm and nucleus. Predicted to be part of transcription regulator complex. Is expressed in several structures, including exocrine cell; intestinal bulb primordium; mid intestine; nervous system; and pancreatic system. Human ortholog(s) of this gene implicated in pancreatic agenesis 2. Orthologous to human PTF1A (pancreas associated transcription factor 1a).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ7ZSX3 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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IHC analysis of PTF1A using anti-PTF1A antibody (AZQ7ZSX3).
PTF1A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTF1A Antibody (AZQ7ZSX3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish PTF1A Antibody (AZQ7ZSX3)
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