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Zebrafish si:dkeyp-75b4.10/eslec Antibody (DZ41383) performs well in IF, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IF analysis of zebrafish eosinophils using Anti-Zebrafish Si:Dkeyp-75b4.10 Antibody (DZ41383).
Si:Dkeyp-75b4.10 was detected in zebrafish eosinophils in FFPE tissue sections and fixed cells. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 3% goat serum in PBS. The tissue section was then incubated with 4 μg/mL rabbit anti-GFAP antibody (MA1045) and anti-MBP antibody (PA1050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126), Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:1000 dilution and incubated for 1 hour at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Si:Dkeyp-75b4.10 was detected in zebrafish eosinophils in FFPE tissue sections and fixed cells. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 3% goat serum in PBS. The tissue section was then incubated with 4 μg/mL rabbit anti-GFAP antibody (MA1045) and anti-MBP antibody (PA1050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126), Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:1000 dilution and incubated for 1 hour at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Zebrafish si:dkeyp-75b4.10/eslec Antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IF, WB |
Tested Applications
antibody was positively detected by WB in:
zebrafish, zebrafish body tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
antibody was positively detected by IF in:
zebrafish eosinophils
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5 μg/ml
Immunofluorescence
4 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish si:dkeyp-75b4.10/eslec Antibody
Form
Liquid
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Observed Molecular Weight
18 kDa
Cite This Product
Anti-Zebrafish si:dkeyp-75b4.10/eslec Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41383)
Protocols
Western Blot protocol for antibody
Immunofluorescence (IF) protocol for antibody
Publications
Application:
Species:
Identification of a Specific Granular Marker of Zebrafish Eosinophils Enables Development of New Tools for Their Study
Published: 2024-10-28
Journal: JOURNAL OF IMMUNOLOGY
Application: IF
Impact Factor: 3.60
Species: Zebrafish
PMID: 39465974
Review
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1 Review For Anti-Zebrafish si:dkeyp-75b4.10/eslec Antibody
Beautiful Imaging of Zebrafish Eosinophils
Source: Biocompare.com
| SKU | DZ41383 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish WKM, IPEX and FFPE tissue sections |
| Primary Incubation | Overnight |
| Blocking Agent | 3% goat serum in PBS |
| Secondary Incubation | 1 hour |
| Tertiary Incubation | None |
| Detection | Fluorescence microscopy |
| DOI or PMID # | https://doi.org/10.1101/2024.04.29.591640 |
| Results Summary | Antibody detects eosinophils and stains eosinophil granula. It is highly specific and can be used in dilutions such as 1:1000 for IF. |
"We ordered this antibody as a custom antibody from Boster Bio to stain for zebrafish eosinophils in FFPE tissue sections and fixed cells. It works wonderfully: very bright, with no relevant background or off-target staining, and highly reproducible."
Miriam Herbert
Verified customer
Submitted 2024-07-03
Product Promise
We promise Anti-Zebrafish si:dkeyp-75b4.10/eslec Antibody performs excellently in relevant applications for IF, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
DZ41383
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 28, 2026