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Product Info Summary
| SKU: | DZ41372 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish sim1a Antibody
SKU/Catalog Number
DZ41372
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Anti-sim1a Antibody catalog # DZ41372. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish sim1a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41372)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Reactive Species
DZ41372 is reactive to in Zebrafish
Observed Molecular Weight
82 kDa
Application & Images
Applications
DZ41372 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5 μg/ml
Positive Control
WB: Zebrafish tissue, Zebrafish head tissue, Zebrafish body tissue
Validation Images & Assay Conditions
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Western blot analysis of Sim1a using anti-Sim1a antibody (DZ41372).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Marker,
Lane 2: Zebrafish tissue lysates,
Lane 3: Zebrafish head tissue lysates,
Lane 4: Zebrafish body tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sim1a antigen affinity purified polyclonal antibody (DZ41372) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sim1a at approximately 82 kDa. The expected band size for Sim1a is at 82 kDa.
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