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Product Info Summary
| SKU: | AZA0ACM8QFG4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish SMAD4a Antibody Picoband®
SKU/Catalog Number
AZA0ACM8QFG4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish SMAD4a Antibody Picoband® catalog # AZA0ACM8QFG4. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish SMAD4a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0ACM8QFG4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish SMAD4a recombinant protein (Position: L153-H270).
Reactive Species
AZA0ACM8QFG4 is reactive to SMAD4a in Zebrafish
Observed Molecular Weight
60 kDa
Background of SMAD4a
Enables sequence-specific DNA binding activity. Contributes to transcription cis-regulatory region binding activity. Acts upstream of or within several processes, including determination of ventral identity; regulation of transmembrane receptor protein serine/threonine kinase signaling pathway; and striated muscle tissue development. Located in nucleus. Part of SMAD protein complex. Is expressed in several structures, including digestive system; head; heart; immature eye; and pleuroperitoneal region. Human ortholog(s) of this gene implicated in several diseases, including female reproductive organ cancer (multiple); hereditary hemorrhagic telangiectasia; juvenile polyposis syndrome; juvenile polyposis-hereditary hemorrhagic telangiectasia syndrome; and seminoma. Orthologous to human SMAD4 (SMAD family member 4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA0ACM8QFG4 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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Western blot analysis of SMAD4a using anti-SMAD4a antibody (AZA0ACM8QFG4).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD4a antigen affinity purified polyclonal antibody (AZA0ACM8QFG4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMAD4a at approximately 60 kDa. The expected band size for SMAD4a is at 55 kDa.
Specific Publications For Anti-Zebrafish SMAD4a Antibody Picoband® (AZA0ACM8QFG4)
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