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Product Info Summary
| SKU: | AZQ6IQ56 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish SNRPD3/3l Antibody Picoband®
SKU/Catalog Number
AZQ6IQ56
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish SNRPD3/3l Antibody Picoband® catalog # AZQ6IQ56. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish SNRPD3/3l Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6IQ56)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish SNRPD3/3l recombinant protein (Position: M1-R112).
Reactive Species
AZQ6IQ56 is reactive to SNRPD3/3l in Zebrafish
Background of SNRPD3/3l
Predicted to enable RNA binding activity. Predicted to be involved in spliceosomal snRNP assembly. Predicted to act upstream of or within RNA splicing and mRNA processing. Predicted to be located in cytosol and nucleus. Predicted to be part of SMN-Sm protein complex; spliceosomal complex; and spliceosomal snRNP complex. Human ortholog(s) of this gene implicated in systemic lupus erythematosus. Orthologous to human SNRPD3 (small nuclear ribonucleoprotein D3 polypeptide).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ6IQ56 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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Western blot analysis of SNRPD3/3I using anti-SNRPD3/3I antibody (AZQ6IQ56).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRPD3/3I antigen affinity purified polyclonal antibody (AZQ6IQ56) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNRPD3/3I at approximately 18 kDa. The expected band size for SNRPD3/3I is at 14 kDa.
Specific Publications For Anti-Zebrafish SNRPD3/3l Antibody Picoband® (AZQ6IQ56)
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