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SOX3 antibody (AZQ6EJB7) performs well in WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of SOX3 using anti-SOX3 antibody (AZQ6EJB7).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: whole female zebrafish tissue lysates,
Lane 2: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX3 antigen affinity purified polyclonal antibody (AZQ6EJB7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SOX3 at approximately 33 kDa. The expected band size for SOX3 is at 33 kDa.
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: whole female zebrafish tissue lysates,
Lane 2: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX3 antigen affinity purified polyclonal antibody (AZQ6EJB7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SOX3 at approximately 33 kDa. The expected band size for SOX3 is at 33 kDa.
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Anti-Zebrafish SOX3 Antibody Picoband®
SOX3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | WB |
Tested Applications
SOX3 antibody was positively detected by WB in:
whole female zebrafish tissue, zebrafish embryo tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish SOX3 Antibody Picoband®
View all SOX3 Antibodies
View all SOX3 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish SOX3 recombinant protein (Position: Q141-Q285)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of SOX3
Enables DNA-binding transcription activator activity, RNA polymerase II-specific. Contributes to DNA-binding transcription factor activity. Involved in regulation of transcription by RNA polymerase II. Acts upstream of or within several processes, including nervous system development; otic placode development; and otic placode formation. Predicted to be active in nucleus. Is expressed in several structures, including ectoderm; nervous system; neural keel; neural tube; and presumptive structure. Human ortholog(s) of this gene implicated in X-linked panhypopituitarism. Orthologous to human SOX3 (SRY-box transcription factor 3).
Cite This Product
Anti-Zebrafish SOX3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6EJB7)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Mouse
74%
Moderate Homology
Rat
74%
Moderate Homology
Rabbit
74%
Moderate Homology
Dog
74%
Moderate Homology
Human
73%
Moderate Homology
Monkey
73%
Moderate Homology
Drosophila
26%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SOX3 antibody
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Product Promise
We promise Anti-Zebrafish SOX3 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZQ6EJB7
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026