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Product Info Summary
| SKU: | AZA0A2R8QRQ5 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish TFAP2A Antibody Picoband®
SKU/Catalog Number
AZA0A2R8QRQ5
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish TFAP2A Antibody Picoband® catalog # AZA0A2R8QRQ5. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish TFAP2A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A2R8QRQ5)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish TFAP2A recombinant protein (Position: M1-K450)
Reactive Species
AZA0A2R8QRQ5 is reactive to TFAP2A in Zebrafish
Background of TFAP2A
Enables DNA-binding transcription factor activity, RNA polymerase II-specific. Acts upstream of or within with a positive effect on melanocyte differentiation. Acts upstream of or within several processes, including embryonic organ morphogenesis; nervous system development; and neural crest cell differentiation. Located in nucleus. Is expressed in several structures, including ectoderm; nervous system; neural crest; neural tube; and pronephros. Human ortholog(s) of this gene implicated in branchiooculofacial syndrome. Orthologous to human TFAP2A (transcription factor AP-2 alpha).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA0A2R8QRQ5 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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Western blot analysis of TFAP2A using anti-TFAP2A antibody (AZA0A2R8QRQ5).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFAP2A antigen affinity purified polyclonal antibody (AZA0A2R8QRQ5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TFAP2A at approximately 50 kDa. The expected band size for TFAP2A is at 50 kDa.
Specific Publications For Anti-Zebrafish TFAP2A Antibody Picoband® (AZA0A2R8QRQ5)
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