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Product Info Summary
| SKU: | AZA0A0R4IMY7 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish TRPM2 Antibody
SKU/Catalog Number
AZA0A0R4IMY7
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish TRPM2 Antibody catalog # AZA0A0R4IMY7. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish TRPM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A0R4IMY7)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish TRPM2 recombinant protein (Position: E54-F1470).
Reactive Species
AZA0A0R4IMY7 is reactive to TRPM2 in Zebrafish
Background of TRPM2
Enables calcium ion binding activity; ligand-gated calcium channel activity; and mono-ADP-D-ribose binding activity. Involved in calcium ion transmembrane transport and protein homotetramerization. Located in plasma membrane. Is expressed in integument; keratinocyte; nervous system; and solid lens vesicle. Orthologous to human TRPM2 (transient receptor potential cation channel subfamily M member 2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA0A0R4IMY7 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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IHC analysis of TRPM2 using anti-TRPM2 antibody (AZA0A0R4IMY7).
TRPM2 was detected in a paraffin-embedded section of zebrafish integument tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRPM2 Antibody (AZA0A0R4IMY7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish TRPM2 Antibody (AZA0A0R4IMY7)
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