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Product Info Summary
| SKU: | AZA0A8M6Z8C1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish TTC28 Antibody
SKU/Catalog Number
AZA0A8M6Z8C1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish TTC28 Antibody catalog # AZA0A8M6Z8C1. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish TTC28 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A8M6Z8C1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish TTC28 recombinant protein (Position: Q1762-D1944).
Reactive Species
AZA0A8M6Z8C1 is reactive to TTC28 in Zebrafish
Background of TTC28
Acts upstream of or within microtubule polymerization or depolymerization. Is expressed in blastomere and nervous system. Orthologous to human TTC28 (tetratricopeptide repeat domain 28).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA0A8M6Z8C1 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Immunofluorescence, 2 μg/ml, Zebrafish
Validation Images & Assay Conditions
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IHC analysis of TTC28 using anti-TTC28 antibody (AZA0A8M6Z8C1).
TTC28 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC28 Antibody (AZA0A8M6Z8C1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of TTC28 using anti-TTC28 antibody (AZA0A8M6Z8C1).
TTC28 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC28 Antibody (AZA0A8M6Z8C1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of TTC28 using anti-TTC28 antibody (AZA0A8M6Z8C1).
TTC28 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC28 Antibody (AZA0A8M6Z8C1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish TTC28 Antibody (AZA0A8M6Z8C1)
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