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th antibody (DZ41285) performs well in IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
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IHC analysis of Tyrosine Hydrxylase/Th using anti-Tyrosine Hydrxylase/Th antibody (DZ41285).
Tyrosine Hydrxylase/Th was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tyrosine Hydrxylase/Th Antibody (DZ41285) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Tyrosine Hydrxylase/Th was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tyrosine Hydrxylase/Th Antibody (DZ41285) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Designing a Western blot for TH? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the TH Western Blot GuideBundle Save
Anti-Zebrafish tyrosine hydrxylase /th Antibody
th antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC, WB |
Tested Applications
th antibody was positively detected by WB in:
Zebrafish tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
th antibody was positively detected by IHC in:
zebrafish cerebellum tissue, zebrafish brain tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5 μg/ml
Immunohistochemistry(Paraffin-embedded Section)
2-5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish tyrosine hydrxylase /th Antibody
View all TH Antibodies
View all TH Antibodies
Form
Liquid
Uniprot
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Observed Molecular Weight
55 kDa
Cite This Product
Anti-Zebrafish tyrosine hydrxylase /th Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41285)
Protocols
Western Blot protocol for th antibody
Immunohistochemistry (IHC) protocol for th antibody
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Product Promise
We promise Anti-Zebrafish tyrosine hydrxylase /th Antibody performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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DZ41285
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 16, 2026