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Product Info Summary
| SKU: | AZA0A0R4ITN2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish Vitamin D binding protein/GC Antibody
SKU/Catalog Number
AZA0A0R4ITN2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish Vitamin D binding protein/GC Antibody catalog # AZA0A0R4ITN2. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish Vitamin D binding protein/GC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A0R4ITN2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish Vitamin D binding protein/GC recombinant protein (Position: D20-A465).
Reactive Species
AZA0A0R4ITN2 is reactive to GC in Zebrafish
Background of GC
Predicted to enable vitamin D binding activity. Predicted to be involved in response to nutrient levels and vitamin D metabolic process. Predicted to be located in extracellular region. Is expressed in hepatocyte; kidney; liver; and testis. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); chronic obstructive pulmonary disease; hepatocellular carcinoma; human immunodeficiency virus infectious disease; and rheumatic fever. Orthologous to human GC (GC vitamin D binding protein).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZA0A0R4ITN2 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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IHC analysis of Vitamin D binding protein/GC using anti-Vitamin D binding protein/GC antibody (AZA0A0R4ITN2).
Vitamin D binding protein/GC was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vitamin D binding protein/GC Antibody (AZA0A0R4ITN2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of Vitamin D binding protein/GC using anti-Vitamin D binding protein/GC antibody (AZA0A0R4ITN2).
Vitamin D binding protein/GC was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vitamin D binding protein/GC Antibody (AZA0A0R4ITN2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish Vitamin D binding protein/GC Antibody (AZA0A0R4ITN2)
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