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GC antibody (AZA0A0R4ITN2) performs well in IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of Vitamin D binding protein/GC using anti-Vitamin D binding protein/GC antibody (AZA0A0R4ITN2).
Vitamin D binding protein/GC was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vitamin D binding protein/GC Antibody (AZA0A0R4ITN2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Vitamin D binding protein/GC was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vitamin D binding protein/GC Antibody (AZA0A0R4ITN2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish Vitamin D binding protein/GC Antibody
GC antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC |
Tested Applications
GC antibody was positively detected by IHC in:
zebrafish kidney tissue, zebrafish liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish Vitamin D binding protein/GC Antibody
View all GC Antibodies
View all GC Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish Vitamin D binding protein/GC recombinant protein (Position: D20-A465).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of GC
Predicted to enable vitamin D binding activity. Predicted to be involved in response to nutrient levels and vitamin D metabolic process. Predicted to be located in extracellular region. Is expressed in hepatocyte; kidney; liver; and testis. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); chronic obstructive pulmonary disease; hepatocellular carcinoma; human immunodeficiency virus infectious disease; and rheumatic fever. Orthologous to human GC (GC vitamin D binding protein).
Cite This Product
Anti-Zebrafish Vitamin D binding protein/GC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A0R4ITN2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Human
32%
Low homology
Rabbit
32%
Low homology
Cow
32%
Low homology
Monkey
31%
Low homology
Sheep
31%
Low homology
Mouse
31%
Low homology
Rat
30%
Low homology
Dog
30%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for GC antibody
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Product Promise
We promise Anti-Zebrafish Vitamin D binding protein/GC Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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AZA0A0R4ITN2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026