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Product Info Summary
| SKU: | AZB5DE31 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish YBX1 Antibody Picoband®
SKU/Catalog Number
AZB5DE31
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish YBX1 Antibody Picoband® catalog # AZB5DE31. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish YBX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZB5DE31)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish YBX1 recombinant protein (Position: R275-D310).
Reactive Species
AZB5DE31 is reactive to YBX1 in Zebrafish
Background of YBX1
Enables several functions, including C5-methylcytidine-containing RNA reader activity; eukaryotic initiation factor 4E binding activity; and methyl-CpG binding activity. Involved in embryonic morphogenesis; mRNA stabilization; and negative regulation of translation. Acts upstream of or within establishment of RNA localization; negative regulation of mRNA splicing, via spliceosome; and negative regulation of translational initiation. Located in P-body and nucleus. Is expressed in blastoderm; blastodisc; blastomere; eye; and telencephalon. Orthologous to several human genes including YBX1 (Y-box binding protein 1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZB5DE31 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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Western blot analysis of YBX1 using anti-YBX1 antibody (AZB5DE31).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: whole female zebrafish tissue lysates,
Lane 3: whole male zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YBX1 antigen affinity purified polyclonal antibody (AZB5DE31) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for YBX1 at approximately 50 kDa. The expected band size for YBX1 is at 36 kDa.
Specific Publications For Anti-Zebrafish YBX1 Antibody Picoband® (AZB5DE31)
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