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Product Info Summary
| SKU: | A11822 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ZFP219 ZNF219 Antibody
SKU/Catalog Number
A11822
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZFP219 ZNF219 Antibody (Catalog # A11822). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ZFP219 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZFP219 ZNF219 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11822)
Host
Rabbit
Contents
ZFP219 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZFP219 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZFP219. The immunogen is located within amino acids 90 - 140 of ZFP219.
Cross-reactivity
At least two isoforms of ZFP219 are known to exist; this antibody will recognize both.
Reactive Species
A11822 is reactive to ZNF219 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
76.9 kDa
Background of ZNF219
ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11822 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZFP219 antibody can be used for detection of ZFP219 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZFP219 in EL4 cell lysate with ZFP219 antibody at 1 μg/mL.
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Immunohistochemistry of ZFP219 in mouse brain tissue with ZFP219 antibody at 5 μg/mL.
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Immunofluorescence of ZFP219 in mouse brain tissue with ZFP219 antibody at 20 μg/mL.
Specific Publications For Anti-ZFP219 ZNF219 Antibody (A11822)
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