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Product Info Summary
| SKU: | A07538 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-ZFP281 ZNF281 Antibody
SKU/Catalog Number
A07538
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZFP281 ZNF281 Antibody (Catalog # A07538). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZFP281 ZNF281 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07538)
Host
Rabbit
Contents
ZFP281 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZFP281 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZFP281. The immunogen is located within amino acids 600 - 650 of ZFP281.
Reactive Species
A07538 is reactive to ZNF281 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
96.9 kDa
Background of ZNF281
ZFP281, also known as ZBP-99, is a member of a conserved family of transcription factors that interact with GC-rich promoters. It contains four Kruppel-like zinc fingers and is highly homologous to ZBP-89, another regulator of ornithine decarboxylase gene expression. ZFP281 also interacts with many other other transcription factors, such as Sp1 to repress vimentin gene transcription. ZFP281 has also been shown to control embryonic stem cell pluripotency by direct regulating specific target genes. ZFP281 phyically interacts with the transcription factos POU5F1, Sox2, and NANOG. Gene expression microarray anlysis indicated that some ZFP281 target genes were activated, while others were repressed upon knockdown of ZFP281 expression, showing that ZFP281 plays bifuctinal roles in regulating gene expression in stem cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07538 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZFP281 antibody can be used for detection of ZFP281 by Western blot at 0.5 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZFP281 in A-20 cell lysate with ZFP281 antibody at 0.5 μg/mL.
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Immunofluorescence of ZFP291 in A20 cells with ZFP291 antibody at 20 μg/mL.
Specific Publications For Anti-ZFP281 ZNF281 Antibody (A07538)
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