Product Info Summary
SKU: | A11198 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ZFYVE19 Antibody
SKU/Catalog Number
A11198
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ZFYVE19 Antibody (Catalog # A11198). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZFYVE19 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11198)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ZFYVE19.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11198 is reactive to Zfyve19 in Human
Applications
A11198 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
43.272kDa
Background of Zfyve19
Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission.
Chinwalla V., Oncogene 22:1400-1410(2003).
Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HT-29 cells, using ZFYVE19 antibody A11198.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using ZFYVE19 antibody A11198.
Protein Target Info & Infographic
Gene/Protein Information For Zfyve19 (Source: Uniprot.org, NCBI)
Gene Name
Zfyve19
Full Name
Abscission/NoCut checkpoint regulator
Weight
43.272kDa
Alternative Names
FLJ14840; MLL partner containing FYVE domain; MPFYVE; zinc finger FYVE domain-containing protein 19; zinc finger, FYVE domain containing 19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Zfyve19, check out the Zfyve19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Zfyve19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZFYVE19 Antibody (A11198)
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No publications found for A11198
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Customer Q&As
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5 Customer Q&As for Anti-ZFYVE19 Antibody
Question
Is a blocking peptide available for product anti-ZFYVE19 antibody (A11198)?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We do provide the blocking peptide for product anti-ZFYVE19 antibody (A11198). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-23
Question
I have a question about product A11198, anti-ZFYVE19 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11198 anti-ZFYVE19 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-27
Question
We are currently using anti-ZFYVE19 antibody A11198 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The anti-ZFYVE19 antibody (A11198) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-18
Question
Would anti-ZFYVE19 antibody A11198 work on bovine WB with liver?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
Our lab technicians have not tested anti-ZFYVE19 antibody A11198 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-ZFYVE19 antibody A11198 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-28
Question
I see that the anti-ZFYVE19 antibody A11198 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-20