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Product Info Summary
| SKU: | M05537 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Zic1 Monoclonal Antibody
SKU/Catalog Number
M05537
BM5282 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Zic1 Monoclonal Antibody catalog # M05537. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Zic1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05537)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACFB-26
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Zic1
Reactive Species
M05537 is reactive to ZIC1 in Human, Mouse, Rat
Observed Molecular Weight
48 kDa
Calculated molecular weight
48.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05537 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: SW480 cell
Validation Images & Assay Conditions
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Western blot analysis of Zic1 expression in SW480 cell lysate.
Specific Publications For Anti-Zic1 Monoclonal Antibody (M05537)
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4 Customer Q&As for Anti-Zic1 Monoclonal Antibody
Question
Does anti-Zic1 Monoclonal antibody M05537 work on feline WB with cerebellum?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
Our lab technicians have not validated anti-Zic1 Monoclonal antibody M05537 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Zic1 Monoclonal antibody M05537 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline cerebellum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-15
Question
I was wanting to use your anti-Zic1 Monoclonal antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
As indicated on the product datasheet, M05537 anti-Zic1 Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-16
Question
We are currently using anti-Zic1 Monoclonal antibody M05537 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
L. Wu
Verified customer
Asked: 2019-11-20
Answer
The anti-Zic1 Monoclonal antibody (M05537) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-20
Question
See attached the WB image, lot number and protocol we used for liver using anti-Zic1 Monoclonal antibody M05537. Please let me know if you require anything else.
L. Taylor
Verified customer
Asked: 2014-08-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-08-27
